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antibodies against evs’ protein markers flotillin-1  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology antibodies against evs’ protein markers flotillin-1
    Characterization of plasma and urine EVs isolated by ExoGAG technology. ( A ) Representative image of NTA profile showing concentration (particles/mL) and size (nm) of plasma EVs (upper panel). Representative profiles of flow cytometry assays for plasma EVs. Alexa 488 fluorescence was used as secondary antibody and non-specific IgG as negative control of fluorescence. Positive <t>CD9</t> and CD63 labelling is represented by a displacement of fluorescence peak curve (lower panels). ( B ) NTA profile of harvested urine EVs expressed as concentration (particles/mL) and size (nm) (upper panel). Flow cytometry analysis of urine EVs resulted in a positive flotillin 1 and CD63 labelling represented by a displacement of fluorescence peak curve (lower panels).
    Antibodies Against Evs’ Protein Markers Flotillin 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flotillin+proteins/anti+cd9/pmc09953104-66-27-30
    Average 90 stars, based on 1 article reviews
    antibodies against evs’ protein markers flotillin-1 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Extracellular Vesicles’ Genetic Cargo as Noninvasive Biomarkers in Cancer: A Pilot Study Using ExoGAG Technology"

    Article Title: Extracellular Vesicles’ Genetic Cargo as Noninvasive Biomarkers in Cancer: A Pilot Study Using ExoGAG Technology

    Journal: Biomedicines

    doi: 10.3390/biomedicines11020404

    Characterization of plasma and urine EVs isolated by ExoGAG technology. ( A ) Representative image of NTA profile showing concentration (particles/mL) and size (nm) of plasma EVs (upper panel). Representative profiles of flow cytometry assays for plasma EVs. Alexa 488 fluorescence was used as secondary antibody and non-specific IgG as negative control of fluorescence. Positive CD9 and CD63 labelling is represented by a displacement of fluorescence peak curve (lower panels). ( B ) NTA profile of harvested urine EVs expressed as concentration (particles/mL) and size (nm) (upper panel). Flow cytometry analysis of urine EVs resulted in a positive flotillin 1 and CD63 labelling represented by a displacement of fluorescence peak curve (lower panels).
    Figure Legend Snippet: Characterization of plasma and urine EVs isolated by ExoGAG technology. ( A ) Representative image of NTA profile showing concentration (particles/mL) and size (nm) of plasma EVs (upper panel). Representative profiles of flow cytometry assays for plasma EVs. Alexa 488 fluorescence was used as secondary antibody and non-specific IgG as negative control of fluorescence. Positive CD9 and CD63 labelling is represented by a displacement of fluorescence peak curve (lower panels). ( B ) NTA profile of harvested urine EVs expressed as concentration (particles/mL) and size (nm) (upper panel). Flow cytometry analysis of urine EVs resulted in a positive flotillin 1 and CD63 labelling represented by a displacement of fluorescence peak curve (lower panels).

    Techniques Used: Clinical Proteomics, Isolation, Concentration Assay, Flow Cytometry, Fluorescence, Negative Control

    Related Articles

    other:

    Article Title: Shiga toxin glycosphingolipid receptors in microvascular and macrovascular endothelial cells: differential association with membrane lipid raft microdomains
    Article Snippet: Human heart whole-cell lysate containing caveolin proteins (Catalog No. ab29431, Abcam, Cambridge, MA) and HeLa whole-cell lysate containing flotillin proteins (Catalog No. sc-2200, Santa Cruz Biotechnology) were used as positive controls.



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    Characterization of plasma and urine EVs isolated by ExoGAG technology. ( A ) Representative image of NTA profile showing concentration (particles/mL) and size (nm) of plasma EVs (upper panel). Representative profiles of flow cytometry assays for plasma EVs. Alexa 488 fluorescence was used as secondary antibody and non-specific IgG as negative control of fluorescence. Positive <t>CD9</t> and CD63 labelling is represented by a displacement of fluorescence peak curve (lower panels). ( B ) NTA profile of harvested urine EVs expressed as concentration (particles/mL) and size (nm) (upper panel). Flow cytometry analysis of urine EVs resulted in a positive flotillin 1 and CD63 labelling represented by a displacement of fluorescence peak curve (lower panels).
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    Image Search Results


    Characterization of plasma and urine EVs isolated by ExoGAG technology. ( A ) Representative image of NTA profile showing concentration (particles/mL) and size (nm) of plasma EVs (upper panel). Representative profiles of flow cytometry assays for plasma EVs. Alexa 488 fluorescence was used as secondary antibody and non-specific IgG as negative control of fluorescence. Positive CD9 and CD63 labelling is represented by a displacement of fluorescence peak curve (lower panels). ( B ) NTA profile of harvested urine EVs expressed as concentration (particles/mL) and size (nm) (upper panel). Flow cytometry analysis of urine EVs resulted in a positive flotillin 1 and CD63 labelling represented by a displacement of fluorescence peak curve (lower panels).

    Journal: Biomedicines

    Article Title: Extracellular Vesicles’ Genetic Cargo as Noninvasive Biomarkers in Cancer: A Pilot Study Using ExoGAG Technology

    doi: 10.3390/biomedicines11020404

    Figure Lengend Snippet: Characterization of plasma and urine EVs isolated by ExoGAG technology. ( A ) Representative image of NTA profile showing concentration (particles/mL) and size (nm) of plasma EVs (upper panel). Representative profiles of flow cytometry assays for plasma EVs. Alexa 488 fluorescence was used as secondary antibody and non-specific IgG as negative control of fluorescence. Positive CD9 and CD63 labelling is represented by a displacement of fluorescence peak curve (lower panels). ( B ) NTA profile of harvested urine EVs expressed as concentration (particles/mL) and size (nm) (upper panel). Flow cytometry analysis of urine EVs resulted in a positive flotillin 1 and CD63 labelling represented by a displacement of fluorescence peak curve (lower panels).

    Article Snippet: The pellet containing EVs was resuspended in 200 μL of PBS and incubated for 1 h at 4 °C with antibodies against EVs’ protein markers [ ]: CD9 (1:50, sc13118, Santa Cruz Biotechnology, Santa Cruz, CA, USA), CD63 (1:50, sc5275, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and flotillin-1 (1:50, sc74566, Santa Cruz Biotechnology, Santa Cruz, CA, USA).

    Techniques: Clinical Proteomics, Isolation, Concentration Assay, Flow Cytometry, Fluorescence, Negative Control

    Characterization of T-AMEVs and B-AMEVs. (A) TEM and (B) LVSEM images of T-AMEVs and B-AMEVs. (C) The size distributions of T-AMEVs and B-AMEVs were evaluated using nanoparticle tracking analysis. The expression of CD9, CD63 and flotillin-2 in T-AMEVs and B-AMEVs was detected by western blotting (D). T-AMEV, tissue adenomyosis-derived extracellular vesicle; B-AMEV, blood adenomyosis-derived extracellular vesicle; TEM, Transmission electron microscopy; LVSEM, low-vacuum scanning electron microscopy; d, diameter; nm, nanometre.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Comparative proteomics identify HSP90A, STIP1 and TAGLN-2 in serum extracellular vesicles as potential circulating biomarkers for human adenomyosis

    doi: 10.3892/etm.2022.11301

    Figure Lengend Snippet: Characterization of T-AMEVs and B-AMEVs. (A) TEM and (B) LVSEM images of T-AMEVs and B-AMEVs. (C) The size distributions of T-AMEVs and B-AMEVs were evaluated using nanoparticle tracking analysis. The expression of CD9, CD63 and flotillin-2 in T-AMEVs and B-AMEVs was detected by western blotting (D). T-AMEV, tissue adenomyosis-derived extracellular vesicle; B-AMEV, blood adenomyosis-derived extracellular vesicle; TEM, Transmission electron microscopy; LVSEM, low-vacuum scanning electron microscopy; d, diameter; nm, nanometre.

    Article Snippet: The separated proteins were transferred to a 0.45-µm PVDF membrane (MilliporeSigma) and blocked in 0.5% bovine serum albumin solution for 30 min at 37˚C, then incubated with anti-flotillin-2 (1:1,000; cat. no. ab181988; Abcam), anti-CD9 (1:2,000; cat. no. ab92726; Abcam), anti-CD63 (1:1,000; cat. no. ab134045; Abcam), anti-HSP90A (1:500; cat. no. AF5368; Affinity Biosciences), anti-STIP1 (1:1,000; cat. no. ab126724; Abcam), anti-TAGLN-2 (1:500; cat. no. AF12053; Affinity Biosciences) and anti-GAPDH (1:1,000; cat. no. E021060-03; EarthOx Life Sciences) antibodies at 4˚C overnight.

    Techniques: Expressing, Western Blot, Derivative Assay, Transmission Assay, Electron Microscopy